Chapters authored
Conservation of Gametes and Use during Assisted Reproductive Technologies in Equine
The appropriate selections, modifications and preservation of gametes are essential for the effectiveness of assisted reproductive technologies (ARTs) in equine. Biotechnologies used in reproduction have an impact on equine production and the preservation of species. In order to address this challenge from a variety of angles and areas, sperm selection techniques, oocyte activation protocols, in vitro fertilization, improvements in oocyte maturation, artificial insemination, embryo transfer, and cryopreservation are all forms of ARTs that, when utilized properly, can help manage and conserve equids. Semen is highly individualistic since no two stallions have the same chemical makeup, which causes each to freeze differently. Other stallions might be able to tolerate the cryopreservation process well, while others might be hypersensitive to it. Since ice crystal formation is uncontrolled in the traditional vapour freezing procedure used to freeze semen straws, the crystals could potentially disturb and harm the sample’s cells. Cryopreserved semen by programmable freezer had higher forward sperm progressive motility than semen frozen in liquid nitrogen vapour, both immediately after thawing and again after 3 hours of incubation. This chapter will present the importance of the selection and manipulation of gametes in equine species.
Part of the book: Equine Science