Chapters authored
The Role of Mesenchymal Stromal Cells in the Management of Osteoarthritis of the Knee
Osteoarthritis (OA) is one of the most common chronic, inflammatory, and degenerative diseases affecting the synovial joints, the hip, and the knee. OA is commonly managed clinically by treating pain with anti-inflammatory medicines using nonsteroidal anti-inflammatory drugs (NSAIDS) or analgesics. In severe OA patients, invasive knee replacement surgery is the last option. Treatment of OA using mesenchymal stromal cells (MSCs) has been widely explored due to their anti-inflammatory properties and chondrogenic differentiation potential. In this chapter, we comprehensively discuss in detail the in vitro OA potency development, OA preclinical studies, and clinical trials conducted using MSCs.
Part of the book: Update on Mesenchymal and Induced Pluripotent Stem Cells
Evaluation and Characterization of Human Bone Marrow Mesenchymal Stromal Cells Cryopreserved in Animal Component-Free, Chemically Defined, Serum-Free Conditions
Mesenchymal stromal cells (MSCs) have the potential to treat various disease indications and are the future of cell therapy-based regenerative medicine. Typically, MSCs cryopreserved in serum-containing freezing formulation are supplied at the clinical site, which necessities that this formulation is removed before the administration. This is a cumbersome process, and there is an immediate need for identifying serum-free, xeno-free cryopreservation medium that can be readily used. Here, we analysed two commercially available serum-free, xeno-free, defined freezing media viz., CryoStor 5 (CS5) and CryoStor 10 (CS10) on their effect on human bone marrow MSCs at different freezing cell densities (5, 10, 12.5, 15 and 25 million cells per ml) over a period of 6 months and compared them to the in-house PlasmaLyte A (PLA)-based cryopreservation media. We found that the MSCs cryopreserved in CS5 and CS10 showed similar characteristics as compared with the in-house freezing media for the various parameters analysed including post-thaw recovery, viability, phenotypic marker expression, CFU-F ability and trilineage differentiation potential of the MSCs. Our results show that human MSC could be successfully cryopreserved using serum-free and xeno-free cryopreservation media and can be delivered to the bedside without any manipulations.
Part of the book: Possibilities and Limitations in Current Translational Stem Cell Research